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KMID : 0364820110470010097
Korean Journal of Microbiology
2011 Volume.47 No. 1 p.97 ~ p.101
Isolation and Characteristics of Endolichenic Fungi Producing Antifungal Compound
Hwang Hyun-Gook

Kim Yi-Na
Baik Keun-Sik
Choi Sang-Ki
Abstract
To isolate a novel antifungal compound, we obtained 107 kinds of endolichenic fungi from Lichen Bioresources Center and examined their antifungal capability. Two fungi EL123 and EL156 showed high antifungal activity against Candida albicans in both MYA and EMM media. Nucleotide sequence analysis and NCBI Blast analysis in ITS region including 5.8S rRNA revealed that EL123 has 95% homology with Thielavia microspora and EL156, 99% with Cryptosporiopsis diversispora which belong to Ascomycetes. It observed that EL156 formed a branched mycelium whereas EL123 formed a
straight one. EL156 also produced the antifungal substance faster than EL123 when they grew on MY liquid medium.
KEYWORD
5.8S rRNA, Cryptosporiopsis diversispora, Thielavia microspora, antifungal, endolichenic fungi
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